An efficient and sustainable intermodal transport of containers requires the optimization of transportation costs and time while minimizing greenhouse gas emissions. For these purposes, within the DBS Gateway Region project, a Pilot Action has been performed focused on the sustainable intermodal transport of containers in the DBS (Danube Black Sea) region. The Pilot Action included two main activities: development of a multi-criteria route optimization application for container transport and tracking and tracing of a container on an intermodal route from China to Serbia to detect the existing bottlenecks and provide recommendations for the development of sustainable intermodal transport in the DBS Gateway Region.